全文获取类型
收费全文 | 3869篇 |
免费 | 401篇 |
国内免费 | 344篇 |
出版年
2024年 | 5篇 |
2023年 | 100篇 |
2022年 | 92篇 |
2021年 | 147篇 |
2020年 | 164篇 |
2019年 | 173篇 |
2018年 | 171篇 |
2017年 | 144篇 |
2016年 | 196篇 |
2015年 | 154篇 |
2014年 | 204篇 |
2013年 | 268篇 |
2012年 | 147篇 |
2011年 | 310篇 |
2010年 | 130篇 |
2009年 | 239篇 |
2008年 | 201篇 |
2007年 | 195篇 |
2006年 | 167篇 |
2005年 | 158篇 |
2004年 | 148篇 |
2003年 | 116篇 |
2002年 | 113篇 |
2001年 | 103篇 |
2000年 | 61篇 |
1999年 | 56篇 |
1998年 | 59篇 |
1997年 | 57篇 |
1996年 | 55篇 |
1995年 | 57篇 |
1994年 | 46篇 |
1993年 | 49篇 |
1992年 | 31篇 |
1991年 | 32篇 |
1990年 | 22篇 |
1989年 | 26篇 |
1988年 | 20篇 |
1987年 | 16篇 |
1986年 | 13篇 |
1985年 | 17篇 |
1984年 | 28篇 |
1983年 | 21篇 |
1982年 | 26篇 |
1981年 | 12篇 |
1980年 | 11篇 |
1979年 | 12篇 |
1977年 | 9篇 |
1976年 | 7篇 |
1975年 | 6篇 |
1973年 | 7篇 |
排序方式: 共有4614条查询结果,搜索用时 140 毫秒
1.
王世荣 《中国微生态学杂志》2015,27(5)
本文阐述了中药防治病毒病的历史及其应用概况,中药联合微生态制剂防治呼吸、消化系统病毒病的理论依据、临床应用和发展前景。 相似文献
2.
A case of chromomycosis in which hyperthermia proved effective is reported. The patient was a 56-year-old male bean curd maker who, without any previous history of minor trauma, developed on the extensor side of the left upper arm an eczematous lesion that underwent gradual radial expansion. The lesion showed a well-defined, 7×10 cm infiltrated erythematous plaque with the central area healed and, at the upper and lower borders, adherent scales and crusts on the surface. Histological examination revealed granulomatous changes in the dermis, as well as sclerotic cells within giant cells and microabscesses. On culturing,Fonsecaea pedrosoi was isolated. The patient was treated with disposable chemical pocket warmers, which were secured over the lesion with a rather tight elastic bandage, so that they kept the affected area warm for 24 hours a day. After a month of such hyperthermic treatment, the erythema and infiltration had decreased considerably, and microscopic examination and culture of the crusts both yielded negative results. Examination of biopsy specimens of the lesion after the third month showed that it had cicatrized. The treatment was stopped after 4 months, and no relapse occurred. We also summarize the published results of local hyperthermic treatment of chromomycosis in Japan. 相似文献
3.
《Cryobiology》2020
Cryopreserved canine adipose-derived mesenchymal stromal cells (Ad-MSCs) can be used instantly in dogs for clinical uses. However, cryopreservation results in a reduction of the cellular viability, proliferation, and anti-oxidation of post-thawed Ad-MSCs. Therefore, there is a need for in-vitro procedure to improve post-thawed Ad-MSCs’ viability, proliferation, anti-oxidation, and differentiation capacity. In this study, fresh-Ad-MSCs were activated with heat shock, hypoxia (5% O2), or hypoxia (5% O2) + heat shock treatments. The results showed that compared to the other treatments, heat shock significantly improved the proliferation rate, anti-oxidation, heat shock proteins and growth factors expressions of canine-fresh-Ad-MSCs. Consequently, fresh-Ad-MSCs were heat-shocked and then cryopreserved with different combinations of dimethyl sulfoxide (Me2SO) and fetal bovine serum (FBS) to determine the combination that could effectively preserve the cellular viability, proliferation, anti-oxidation and differentiation capacity of Ad-MSCs after cryopreservation. We found that C-HST-Ad-MSCs cryopreserved with 10% Me2SO + 40% FBS presented significantly (p < 0.05) improved cellular viability, proliferation rate, anti-oxidant capacity, and differentiation potential as compared to C-HST-Ad-MSCs cryopreserved with 1% Me2SO + 10% FBS or 1% Me2SO alone or control. We concluded, heat shock treatment is much better to enhance the characteristics of fresh-Ad-MSCs than other treatments, moreover, C-HST-Ad-MSCs in 10% Me2SO + 40% FBS showed better results compared to other cryopreserved groups. However, future work is required to optimize the expression of heat shock proteins, which would further improve the characteristics of fresh- and cryopreserved-HST-Ad-MSCs and reduce the dependency on Me2SO and FBS. 相似文献
4.
Dr. Jan Lanke 《Biometrical journal. Biometrische Zeitschrift》1987,29(3):277-286
For patients of a certain type, a number of treatments are available. The effect of each such treatment is assumed to be described by a shift model; it is, however, admitted that there may be an interaction between patient and treatment, meaning in particular that the treatment which is best for one patient is not necessarily best for another. The problem is the following: if each patient is given the treatment which is optimal for that particular patient, will this produce a significant effect and, if so, how large is the effect? 相似文献
5.
Impacts of sheep ticks Ixodes ricinus on livestock, gamebirds and wildlife are of concern across Europe. The present study describes livestock and tick management by 36 farmers from three upland sites of conservation importance in North Wales, where farmers consider that ticks have increased during the last 25 years. Sheep, average densities of 2.0 animals per ha were treated with pour‐on acaricides in spring, again in July, and also when removed from the moor in autumn. Given acaricide efficacy rates, sheep were susceptible to tick bites for half the period on the moor. Sheep from 17 farms were examined for ticks. Infestations were similar between farms and in relation to the acaricide used, averaging 9.3 ticks per sheep, although they were lower where the interval between successive acaricide treatments was shorter. Repeated sampling of sheep and red grouse chicks showed no annual difference in tick burdens on grouse chicks, which averaged 6.2 ticks per chick, although there were three‐fold fewer ticks on sheep in 2018 than in previous years. Tick bite rates on sheep and grouse were higher than elsewhere in the U.K. Most farmers interviewed would aim to improve their tick management using longer‐lasting acaricides and treating sheep more frequently, although they would need advice and financial help, which is currently unavailable via Government funded agri‐environment schemes. 相似文献
6.
E. C. Jorgenson 《Journal of nematology》1979,11(2):144-150
Growth and yield of cotton were best with combinations of fumigants and organophosphate and carbamate nematicides. Organophosphates or carbamates used alone did not give season-long control of root-knot nematodes. Long-term control was poor because the temporary sublethal effects of these materials diminished soon enough lhat the nematodes could reproduce. The nematodes survived the treatments and a year of nonhost culture, and damaged a susceptible host crop 2 years after treatment. No such damage occurred in plots treated with fumigant, fumigant plus organophosphate, or fumigant plus carbamate. Treatment of seed and treatment of cotton, either in furrow at planting or sidedressing at midseason, with organophosphate and carbamate nematicides resulted in little or no yield increase, because nematode control was only minimal and temporary; or in a yield decrease, because the toxicity of the materials was manifested when nematode populations were low. 相似文献
7.
B M Anner 《FEBS letters》1983,158(1):7-11
Purified Na+,K+-ATPase is treated with trypsin. The altered enzyme is then reconstituted into liposomes and the change in active and passive Na+,K+-fluxes is recorded. Trypsin treatment transforms the slow passive Na+,K+-fluxes into leaks. The leak formation is correlated with the degree of proteolysis and the associated decrease in Na+,K+-ATPase activity. The active Na+,K+-transport capacity decreases in parallel with the passive transport. It is thus proposed that the Na+,K+-ATPase molecule primarily contains unspecific transmembrane tunnels that are rendered ion-selective by transverse bars of specific length (bar model). 相似文献
8.
C.S. Fagnant-Sperati Y. Ren N.A. Zhou E. Komen B. Mwangi J. Hassan A. Chepkurui R. Nzunza J. Nyangao W.B. van Zyl M. Wolfaardt P.N. Matsapola F.B. Ngwana S. Jeffries-Miles A. Coulliette-Salmond S. Peñaranda E. Vega J.H. Shirai A.L. Kossik N.K. Beck D.S. Boyle C.C. Burns M.B. Taylor P. Borus J.S. Meschke 《Journal of applied microbiology》2021,130(3):971-981
9.
The study investigated the in vitro, ex vivo and in vivo efficacy of ajoene and ciprofloxacin (CIP) alone and in combination against Pseudomonas aeruginosa biofilms and biofilm-associated murine acute pyelonephritis. The ajoene–CIP combination exhibited significant greater (p < 0.05) antimotility and biofilm inhibitory effects than those obtained when they were applied individually. The combined action of the agents resulted in a significant increase in serum sensitivity and phagocytic uptake and killing of P. aeruginosa (p < 0.001) compared to the untreated control. Mice groups treated with an ajoene (25 mg kg?1) and CIP (30 mg kg?1 or 15 mg kg?1) combination showed a significantly (p < 0.001) reduced bacterial load in the kidney and bladder as compared to that of infected controls and mice treated with solo agents on the fifth day post-infection. The decreased levels of biomarkers and photomicrographs of the kidney tissue of the treated mice showed a reduced severity of damage. Hence, the study highlights the antivirulent and therapeutic efficacy of the ajoene-CIP combination at the minimal dosage of CIP. 相似文献
10.
Surface properties of Sendai virus envelope membrane have been measured, using both biological and biophysical techniques. Both normal and trypsin-treated virus were studied. SDS gel electrophoresis showed cleavage of the F protein exclusively by trypsin. The major activity change was observed in the hemolysing activity which is an expression of F protein. Hemolysis was reduced to less than 10% of its value for intact virus. 31P nuclear magnetic resonance studies of the envelope surface of the native virus showed a highly restricted phospholipid headgroup environment. Interestingly, this restriction was relieved by treatment with trypsin. Thus these data suggest a role of the F protein of Sendai virus in tightly organizing the surface of the viral envelope membrane. 相似文献